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朱作言文集（全二册） 
定 价：880 元
9 7 6 8 0 7 4 0 6 3 1 0 3 读者对象:本书适用于鱼类学、鱼类生理学、鱼类内分泌学、农业动物基因工程育种等领域的科技工作者 
朱作言先生主要从事鱼类遗传发育生物学及生物技术方面的研究。本书为其多年著作的合集，分别从鱼类基因转移模型建立、鱼类外源基因的整合与表达、外源基因表达的生理功能、转基因黄河鲤的生态安全研究、鱼类生殖调控与转基因鱼的育性控制研究、抗病草鱼基因工程技术育种研究、鱼类基因转移育种技术展望这几个部分进行整理。
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目 录
上 卷 第一部分 鱼类基因转移模型建立 Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3 人生长激素基因在泥鳅受精卵显微注射转移后的生物学效应 8 Introduction of Novel Genes into Fish 11 转基因鱼模型的建立 23 第二部分 外源基因的整合与表达 The β-Actin Gene of Carp (Ctenopharyngodon idella) 35 Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37 Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from Grass Carp (Ctenopharyngodon idellus) 54 Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64 Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are Highly Polymorphic 73 Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84 Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp (Cyprinus carpio L ) 87 Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the Selection of Site-Directed Knockout/Knockin 101 Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase Using A Combination of Mutant Lox Sites 114 Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence (EGS) Technique 128 Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation in Transgenic Zebrafish Embryos 137 Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157 Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia (Oreochromis niloticus) 172 Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UAS Systems 189 Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in Zebrafish Using TALENs 211 Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNA Double-Strand Breaks in Zebrafish Embryos 225 第三部分 外源基因表达的生理功能 Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function in Hypophysectomized Carp 249 Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinus carpio L red var ) 263 Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic Red Common Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269 Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes Thymus Development 276 Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-Specific Immune Functions of Common Carp, Cyprinus carpio L 287 Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: Reduced Critical Swimming Speed 298 Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic Common Carp 307 Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321 Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus carpio L ) 334 Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of Growth Hormone (cGH) in Common Carps (Cyprinus carpio) 350 The Hematological Response to Exhaustive Exercise in ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus carpio L ) 362 Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus carpio L ) 376 Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone Development in Common Carp 386 Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L ) May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401 第四部分 转基因黄河鲤的生态安全研究 Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and Its Containment Strategies 423 Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpio Associated with Their Morphological Variations 433 Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ Growth Hormone Transgenic Common Carp Cyprinus carpio 446 Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L ) Under Short-Term Predation Risk 462 Behavioral Alterations in GH Transgenic Common Carp May Explain Enhanced Competitive Feeding Ability 471 Risk-Taking Behaviour May Explain High Predation Mortality of GH-Transgenic Common Carp Cyprinus carpio 489 Transgenic Common Carp Do Not Have the Ability to Expand Populations 506 Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio: Implications for Environmental Risk 518 第五部分 鱼类生殖调控与转基因鱼的育性控制研究 A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535 Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH (cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546 Identification and Characterization of A Novel Splice Variant of Gonadotropin α Subunit in the Common Carp Cyprinus carpio 560 Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin Synthesis and Gonadal Development in Transgenic Common Carp (Cyprinus carpio) 574 Production, Characterization, and Applications of Mouse Monoclonal Antibodies Against Gonadotropin, Somatolactin, and Prolactin from Common Carp (Cyprinus carpio) 588 Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio) 601 Rapid Growth and Sterility of Growth Hormone Gene Transgenic Triploid Carp 625 Progress in Studies of Fish Reproductive Development Regulation 636 Effects of Growth Hormone Over-Expression on Reproduction in the Common Carp Cyprinus carpio L 655 Endocrinology: Advances Through Omics and Related Technologies 678 A Controllable On-Off Strategy for the Reproductive Containment of Fish 706 The Distribution of Kisspeptin (Kiss)1- and Kiss2-Postive Neurones and Their Connections with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain 726 Direct Production of XYDMY– Sex Reversal Female Medaka (Oryzias latipes) by Embryo Microinjection of TALENs 749 下 卷 第六部分 抗病草鱼基因工程技术育种研究 Sequence of Genome Segments 1, 2, and 3 of the Grass Carp Reovirus (Genus Aquareovirus, Family Reoviridae) 775 Genome Segment S8 of Grass Carp Hemorrhage Virus Encodes A Virion Protein 783 Molecular Characterization and Expression of the M6 Gene of Grass Carp Hemorrhage Virus (GCHV), An Aquareovirus 789 Complete Nucleotide Sequence of the S10 Genome Segment of Grass Carp Reovirus (GCRV) 796 Introduction of the Human Lactoferrin Gene into Grass Carp (Ctenopharyngodon idellus) to Increase Resistance Against GCH Virus 806 Enhanced Resistance to Aeromonas hydrophila Infection and Enhanced Phagocytic Activities in Human Lactoferrin-Transgenic Grass Carp (Ctenopharyngodon idellus) 816 Molecular Cloning, Characterization and Expression Analysis of the PKZ Gene in Rare Minnow Gobiocypris rarus 827 Toll-Like Receptor 3 Regulates Mx Expression in Rare Minnow Gobiocypris rarus After Viral Infection 842 Enhanced Grass Carp Reovirus Resistance of Mx-Transgenic Rare Minnow (Gobiocypris rarus) 861 Isolation and Characterization of Argonaute 2: A Key Gene of the RNA Interference Pathway in the Rare Minnow, Gobiocypris rarus 877 Transcriptome Analysis of Head Kidney in Grass Carp and Discovery of Immune-Related Genes 892 Cloning and Characterization of the Grass Carp (Ctenopharyngodon idella) Toll-Like Receptor 22 Gene, A Fish-Specific Gene 911 Identification, Characterization and the Interaction of Tollip and IRAK-1 in Grass Carp (Ctenopharyngodon idellus) 930 Isolation and Analysis of A Novel Grass Carp Toll-Like Receptor 4 (tlr4) Gene Cluster Involved in the Response to Grass Carp Reovirus 950 Cloning and Preliminary Functional Studies of the JAM-A Gene in Grass Carp (Ctenopharyngodon idellus) 962 RNA-Seq Profiles from Grass Carp Tissues After Reovirus (GCRV) Infection Based on Singular and Modular Enrichment Analyses 980 Isolation and Expression of Grass Carp Toll-Like Receptor 5a (CiTLR5a) and 5b (CiTLR5b) Gene Involved in the Response to Flagellin Stimulation and Grass Carp Reovirus Infection 1001 Characterizations of Four Toll-Like Receptor 4s in Grass Carp Ctenopharyngodon idellus and Their Response to Grass Carp Reovirus Infection and Lipopolysaccharide Stimulation 1022 The Draft Genome of the Grass Carp (Ctenopharyngodon idellus) Provides Insights into Its Evolution and Vegetarian Adaptation 1035 第七部分 鱼类基因转移育种技术展望 Embryonic and Genetic Manipulation in Fish 1057 Nuclear Transplantation with Early-Embryonic Cells of Transgenic Fish 1067 The Onset of Foreign Gene Transcription in Nuclear-Transferred Embryos of Fish 1075 Nuclear Transplantation of Somatic Cells of Transgenic Red Carp (Cyprinus carpio haematopterus) 1086 Nuclear Transplantation in Different Strains of Zebrafish 1095 Cytoplasmic Impact on Cross-Genus Cloned Fish Derived from Transgenic Common Carp (Cyprinus carpio) Nuclei and Goldfish (Carassius auratus) Enucleated Eggs 1103 Identification of Differentially Expressed Genes from the Cross-Subfamily Cloned Embryos Derived from Zebrafish Nuclei and Rare Minnow Enucleated Eggs 1117 Identification and Characterization of A Novel Gene Differentially Expressed in Zebrafish Cross-Subfamily Cloned Embryos 1132 Identification of Differential Transcript Profiles Between Mutual Crossbred Embryos of Zebrafish (Danio rerio) and Chinese Rare Minnow (Gobiocypris rarus) by cDNA-AFLP 1146 Identification of A Novel Gene K23 Over-Expressed in Fish Cross-Subfamily Cloned Embryos 1162 Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization 1171 Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in Zebrafish 1196 Cross-Species Cloning: Influence of Cytoplasmic Factors on Development 1212 Efficient RNA Interference in Zebrafish Embryos Using siRNA Synthesized with SP6 RNA Polymerase 1219 Cloning, Characterization and Promoter Analysis of Common Carp Hairy/Enhancer-of-Split-Related Gene, her6 1233 Knock Down of gfp and No Tail Expression in Zebrafish Embryo by In Vivo-Transcribed Short Hairpin RNA with T7 Plasmid System 1246 Hybrid Cytomegalovirus-U6 Promoter-Based Plasmid Vectors Improve Efficiency of RNA Interference in Zebrafish 1260 The Cytomegalovirus Promoter-Driven Short Hairpin RNA Constructs Mediate Effective RNA Interference in Zebrafish In Vivo 1272 Molecular Cloning of Growth Hormone Receptor (GHR) from Common Carp (Cyprinus carpio L ) and Identification of Its Two Forms of mRNA Transcripts 1286 Comparative Expression Analysis of GHR Signaling Related Factors in Zebrafish (Danio rerio) and An In Vivo Model to Study GHR Signaling 1298 Activation of GH Signaling and GH-Independent Stimulation of Growth in Zebrafish by Introduction of A Constitutively Activated GHR Construct 1318 Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio) 1335 Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3 Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model 1350 Developments in Transgenic Fish in the People’s Republic of China 1374 Father of Biological Cloning in China 1386 Fish Genome Manipulation and Directional Breeding 1389 后记 1403
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